| 論文編號: |
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| 第一作者所在部門: |
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| 中文論文題目: |
Low glutaminase and glycolysis correlate with a high transdifferentiation efficiency in mouse cortex |
| 英文論文題目: |
Low glutaminase and glycolysis correlate with a high transdifferentiation efficiency in mouse cortex |
| 作者: |
Li, Yuan; Yang, Tingting; Cheng, Yingying; Hou, Jinfei; Liu, Zhaoming; Zhao, Yu; Chen, Shiyu; Qin, Zhaohui; Wang, Chenchen; Song, Weining; Ge, Haofei; Li, Changpeng; Liang, Lining; Guo, Lin; Sun, Hao; Wu, Lin-Ping; Zheng, Hui |
| 論文出處: |
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| 刊物名稱: |
CELL PROLIFERATION |
| 年: |
2023 |
| 卷: |
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| 期: |
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| 頁: |
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| 聯係作者: |
Zheng, Hui |
| 收錄類別: |
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| 影響因子: |
8.755 |
| 摘要: |
Both exogenous transcriptional factors and chemical-defined medium can transdifferentiate astrocytes into functional neurons. However, the regional preference for such transdifferentiation has not been fully studied. A previously reported 5C medium was infused into the mouse cortex and striatum to determine the regional preference for transdifferentiation from astrocytes to neurons. The numbers of NeuN(+)GFAP(+)EdU(+) cells (intermediates) and NeuN(+)EdU(+) cells (end products) were determined by immunofluorescence to explore the regional preference of transdifferentiation. In addition, to optimize the delivery of the transdifferentiation medium, three key growth factors, insulin, bFGF and transferrin, were loaded onto chitosan nanoparticles, mixed with gelatin methacryloyl and tested in animals with motor cortex injury. A higher transdifferentiation efficiency was identified in the mouse cortex. Differences in cellular respiration and the balance between glutaminase (Gls) and glutamine synthetase were confirmed to be key regulators. In addition, the sustained drug release system induced transdifferentiation of cortex astrocytes both in vivo and in vitro, and partially facilitated the behaviour recovery of mice with motor cortex injury. We also applied this method in pigs and obtained consistent results. In summary, low Gls and glycolysis can be used to predict high transdifferentiation efficiency, which may be useful to identify better indications for the current transdifferentiation system. In addition, the current drug delivery system has the potential to treat diseases related to cortex injuries. |
| 英文摘要: |
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