2002.09-2006.07青島農業大學 動物醫學學士

2006.09-2009.07華南農業大學 臨床獸醫學碩士

2009/07—至今 betway体育下载 助理研究員，高級實驗師

基因編輯動物模型的建立及其行為學和影像學分析與幹細胞及基因治療研究；

褪黑素和氨基胍對內毒素血症山羊心肌及其線粒體細胞凋亡水平的影響

以CRISPR/Cas9係統打靶PINK/PARK雙基因製備人類帕金森疾病豬模型，2016A030313169，廣東省自然科學基金，主持

老年癡呆豬模型發病早期中期晚期行為學及核磁影像學評價標準的建立與幹細胞治療，2019A030317010，廣東省自然科學基金，主持

參與獲得廣州市科技進步1等獎；

參與獲得廣東省科技進步1等獎；

betway体育下载 先進工作者；

國家首批執業獸醫師；

南粵優秀研究生；

山東省省級三好學生；

山東省省級優秀畢業生；

1.Yan S^#, Tu Z^#,Liu Z^#（共同一作）, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C, Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S^＊,Lai L^＊,Li XJ^＊.A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease.Cell.2018 May 3;173(4):989-1002.

2．Yang D^#, Yang H^#, Li W, Zhao B, Ouyang Z,Liu Z,Zhao Y, Fan N, Song J, Tian J, Li F, Zhang J, Chang L, Pei D, Chen YE^＊,Lai L^＊.Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning.Cell Res.2011Jun;21(6):979-82.

3．Yang H^#, Wang G^#, Sun H^#, Shu R^#, Liu T, Wang CE,Liu Z,Zhao Y, Zhao B, Ouyang Z, Yang D, Huang J, Zhou Y, Li S, Jiang X^＊, Xiao Z^＊, Li XJ^＊,Lai L^＊.Species-dependent neuropathology in transgenic SOD1 pigs.Cell Res.2014 Apr;24(4):464-81.

4．Li X^#, Yang Y^#, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z,Liu Z,Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z^＊,Lai L^＊.Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing.Cell Res.2014 Apr;24(4):501-4. Epub 2014 Feb 7.

5．Fan N^#, Chen J^#, Shang Z^#, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N, Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z,Liu Z,Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y^＊, Xiao L^＊,Lai L^＊.Piglets cloned from induced pluripotent stem cells.Cell Res.2013 Jan;23(1):162-6.

6.Xie J^#, Ge W^#, Li N^#, Liu Q^#, Chen F, Yang X, Huang X, Ouyang Z, Zhang Q, Zhao Y, Liu Z, Gou S, Wu H, Lai C, Fan N, Jin Q, Shi H, Liang Y, Lan T, Quan L, Li X, Wang K^＊, Lai L^＊. Efficient base editing for multiple genes and loci in pigs using base editors.Nat Commun.2019 Jun 28;10(1):2852.

7．Wang G^#, Yang H^#, Yan S^#, Wang CE, Liu X, Zhao B, Ouyang Z, Yin P,Liu Z,Zhao Y, Liu T, Fan N, Guo L, Li S^＊, Li XJ^＊,Lai L^＊.Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain.Mol Neurodegener.2015 Sep 3;10:42.

8.Wang K^#, Jin Q^#, Ruan D^#, Yang Y, Liu Q, Wu H, Zhou Z, Ouyang Z,Liu Z,Zhao Y, Zhao B, Zhang Q, Peng J, Lai C, Fan N, Liang Y, Lan T, Li N, Wang X, Wang X, Fan Y, Doevendans PA, Sluijter JPG, Liu P^＊, Li X^＊,Lai L^＊.Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.Genome Res.2017 Dec;27(12):2061-2071.

9.Wu H^#, Liu Q^#, Shi H, Xie J, Zhang Q, Ouyang Z, Li N, Yang Y,Liu Z,Zhao Y, Lai C, Ruan D, Peng J, Ge W, Chen F, Fan N, Jin Q, Liang Y, Lan T, Yang X, Wang X, Lei Z, Doevendans PA, Sluijter JPG, Wang K^＊, Li X^＊,Lai L^＊.Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems.Cell Mol Life Sci.2018 Oct;75(19):3593-3607.

10．Zhou X^#, Xin J^#, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B,Liu Z,Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H^＊,Lai L^＊.Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer.Cell Mol Life Sci.2015 Mar;72(6):1175-84. Epub 2014 Oct 2.

11.Ruan D^#, Peng J^#, Wang X^#, Ouyang Z^#, Zou Q, Yang Y, Chen F, Ge W, Wu H,Liu Z,Zhao Y, Zhao B, Zhang Q, Lai C, Fan N, Zhou Z, Liu Q, Li N, Jin Q, Shi H, Xie J, Song H, Yang X, Chen J, Wang K^＊, Li X^＊,Lai L^＊.XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer.Stem Cell Reports.2018 Feb 13;10(2):494-508.

12.Jin Q, Yang X, Gou S, Liu X, Zhuang Z, Liang Y, Shi H, Huang J, Wu H, Zhao Y, Ouyang Z, Zhang Q, Liu Z, Chen F, Ge W, Xie J, Li N, Lai C, Zhao X, Wang J, Lian M, Li L, Quan L, Ye Y, Lai L, Wang K. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Sci China Life Sci. 2022 May 18.

13.Yang Y^#, Wang K^#, Wu H, Jin Q, Ruan D, Ouyang Z, Zhao B,Liu Z,Zhao Y, Zhang Q, Fan N, Liu Q, Guo S, Bu L, Fan Y, Sun X, Li X^＊,Lai L^＊.Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering.J Mol Cell Biol.2016 Apr;8(2):174-7.

14．Zou Q^#, Wang X^#, Liu Y^#, Ouyang Z^#, Long H, Wei S, Xin J, Zhao B, Lai S, Shen J, Ni Q, Yang H, Zhong H, Li L, Hu M, Zhang Q, Zhou Z, He J, Yan Q, Fan N, Zhao Y,Liu Z,Guo L, Huang J, Zhang G, Ying J^＊,Lai L^＊,Gao X^＊. Generation of gene-target dogs using CRISPR/Cas9 system.J Mol Cell Biol.2015 Dec;7(6):580-3. doi: 10.1093

15．Yang D^#, Wang CE^#, Zhao B, Li W, Ouyang Z,Liu Z,Yang H, Fan P, O'Neill A, Gu W, Yi H, Li S,Lai L^＊,Li XJ^＊. Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs.Hum Mol Genet.19 (20): 3983-94.

16.Han K^#, Liang L^#, Li L^#, Ouyang Z, Zhao B, Wang Q,Liu Z,Zhao Y, Ren X, Jiang F, Lai C, Wang K, Yan S, Huang L, Guo L, Zeng K^＊,Lai L^＊,Fan N^＊.Generation of Hoxc13 knockout pigs recapitulates human ectodermal dysplasia-9.Hum Mol Genet.2017 Jan 1;26(1):184-191.

17.Huang J^#, Guo X^#, Fan N, Song J, Zhao B, Ouyang Z,Liu Z,Zhao Y, Yan Q, Yi X, Schambach A, Frampton J, Esteban MA, Yang D, Yang H^＊,Lai L＊.RAG1/2 knockout pigs with severe combined immunodeficiency.J Immunol.2014 Aug 1;193(3): 1496-503.

18.Li W^#, Shi L^#, Zhuang Z^#, Wu H^#, Lian M, Chen Y, Li L, Ge W, Jin Q, Zhang Q, Zhao Y, Liu Z, Ouyang Z, Ye Y, Li Y, Wang H, Liao Y, Quan L, Xiao L, Lai L^＊, Meng G^＊, Wang K^＊.Engineered Pigs Carrying a Gain-of-Function NLRP3 Homozygous Mutation Can Survive to Adulthood and Accurately Recapitulate Human Systemic Spontaneous Inflammatory Responses.J Immunol.2020 Nov 1;205(9):2532-2544. doi:10.4049/jimmunol.1901468. Epub 2020 Sep 21.PMID: 32958688

19.Lan T^#, Zheng Y^#, Suo Y, Wei Y, Shi H, Yan Q, Zhuang Z, Chen H, Zhang Q, Fan N, Zhao Y, Ouyang Z, Lai C,Liu Z,Zhou J, Tang C, Kim NH, Zou Q^＊, Wang X^＊. Generation of pigs with humanized type II collagen by precise human COL2A1 gene knock-in.J Genet Genomics. 2022 May 19:S1673-8527(22)00139-4.

20.Chen P^#, Gao H^#, Lu Y, Nie H,Liu Z,Zhao Y, Fan N, Zou Q, Dai Y, Tang A, Hara H, Cai Z, Cooper DKC,Lai L＊,Mou L^＊.Altered expression of eNOS, prostacyclin synthase, prostaglandin G/H synthase, and thromboxane synthase in porcine aortic endothelial cells after exposure to human serum-relevance to xenotransplantation.Cell Biol Int.2017 Jul;41(7):798-808.

21．Lai S^#, Wei S, Zhao B, Ouyang Z, Zhang Q, Fan N,Liu Z,Zhao Y, Yan Q, Zhou X, Li L, Xin J, Zeng Y^＊,Lai L^＊,Zou Q.Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering.PLoS One.2016 Jan 12;11(1):e0146562.

22．Deng W^#, Yang D^#, Zhao B, Ouyang Z, Song J, Fan N,Liu Z,Zhao Y, Wu Q, Nashun B, Tang J, Wu Z, Gu W^＊,Lai L^＊.Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer.PLoS One.2011;6(5):e19986. Epub 2011 May 13.

23.Xin J^#, Yang H^#, Fan N, Zhao B, Ouyang Z,Liu Z,Zhao Y, Li X, Song J, Yang Y, Zou Q, Yan Q, Zeng Y^＊,Lai L^＊.Highly Efficient Generation of GGTA1 Biallelic Knockout Inbred Mini-Pigs with TALENs.PLoS One.2013 Dec 178(12)

24.Yong Wang^#, Hua-Qiang Yang^#, Wen Jiang, Na-Na Fan, Ben-Tian Zhao, Zhen Ou-Yang,Zhao-Ming Liu,Yu Zhao, Dong-Shan Yang, Xiao-Yang Zhou, Hai-Tao Shang, Lu-Lu Wang, Peng-Ying Xiang, Liang-Peng Ge, Hong Wei^＊, Liang-Xue Lai^＊. Transgenic expression of human cytoxic T-lymphocyte associated antigen4-immunoglobulin (hCTLA4Ig) by porcine skin for xenogeneic skin grafting.Transgenic Res.2015 Apr;24(2):199-211.

25.Yan Q^#, Yang H, Yang D, Zhao B, Ouyang Z,Liu Z,Fan N, Ouyang H, Gu W,Lai L^＊.Production of transgenic pigs over-expressing the antiviral gene Mx1.Cell Regen .2014 Sep 26;3(1)

26．Liu Zhao-ming，Wu Caixia，Yang Hong, Li Ying,Pan Jia-qiang,Li Peng-fei,Guo Ming-sheng,Liu nian,Zeng Jian-ying,Tang Zhao-xin^＊.Protective Effects of Melatonin on Myocardial No Metabolism and Sulfhydryl in Goats with Endotoxemia.中國首屆獸醫臨床大會暨中國畜牧獸醫學年會優秀論文獎.

27．LIU Zhao-ming,YANG Hong,SHI Da-you,LIU Jing,GUO Jian-ying,LI Ying,PAN Jia-qiang,TANG Zhao-xin^＊.Protective effects of aminoguanidin on Myocardial mitochondrial lipid peroxidation damage and the activity of ATPase in goats with endotoxemia.中國第二屆獸醫臨床大會暨中國畜牧獸醫學內科年會優秀論文獎.